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ABSTRACT: Benzothiazoles and pyrazoles moieties structurally have better anti inflammatory activity. Therefore various of-chloro-7-fluoro-1-[4-methylphenyl] sulphonyl 1,9 dihydro[1,2,4] triazol[3,4-b][1,3] Benzothiazole containing different functional groups have been synthesized by condensing 7- Chloro-6-Fluoro-2-amino-Benzothiazole with hydrazine hydrate in the presence of ethylene glycol and conc. HCl to get 7-chloro-6-fluoro-2-hydrazinyl-1, 3-benzothiazole and then treated with potassium carbonate to get 8-chloro-7-fluoro-1,9a-dihydrol [1,2,4] triazole [3,4-b][1,3] benzothiazole and then treated with p-toulene sulphonamide in the presence of pyridine to get 8-chloro-7-fluoro-1-[4-methylphenyl] sulphonyl-1,9a-dihydro [1,2,4]triazolo [3,4-b][1,3]benzothiazole. To the above product different aromatic amines, as well as various primary and secondary amines in presence of DMF were treated to get newly targeted compounds by replacing chlorine at 7th position. The characterization of the compounds were confirmed on the basis of their spectral (IR, 1H-NMR and MASS) data. Compounds (TZ1-TZ9) synthesized are screened for anti oxidant activity. These compounds are screened for the activity at 5 mg/ml using Ascorbic acid as standard. The percentage inhibition of the compounds at various concentrations is calculated from their absorbance values. Among the screened compounds, TZ-5 showed potent activity against the standard. Where as compounds TZ-7, TZ-8, TZ-4 showed moderate activity and compound TZ-1 was less active than the standard.
KeyWords: Benzothiazoles, triazoles, antioxidant, DPPH.
How to cite: Naresh P et. al. Synthetic Characterization and Antioxidant Screening of Some Novel 6-Fluorobenzothiazole Substituted [1, 2, 4] Triazole Analogues. Int J Pharma Sci. 3(2) 2013: 170-174
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170-174
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ABSTRACT: It was found that there were two new water sources (Ain Alhaya and Ain Alkhiala). Ain Alhaya is one of the most common springs in Cairo. The source of this water is the deep meteoric water. Ain El-Khiala is a spring located to the south of Ain Alhaya. The source of water at this spring is the shallow marine water and characterized by greenish brown colour. Water of Ain Alkhiala was characterized by lower dissolved oxygen and higher levels of turbidity, conductivity, sulfates and ammonia than that of Ain Alhaya. It was also characterized by the presence of wide range of different algae and protozoa species. So, water of Ain Alkhiala was excluded from the treatment. Although the flocculation with chlorine was more effective than that with huwa-san, the disinfection with huwa-san was more effective in killing the different bacterial and protozoa species than chlorine in both of Ain Alhaya and Ain Alkhiala. The study at the chemical and microbiological levels showed that water of Ain Alhaya may be suitable for drinking purposes after further experimental studies for removing the metals which were responsible for the high conductivity. Huwa-san may be the most suitable disinfecting agent used during treatment of water of Ain Alhaya.
KeyWords: Ain Alhaya, Ain Alkiala, Disinfection, Chlorine, Huwa-san
How to cite: Hussein MA et al. Evaluation of new water sources to be suitable for drinking purposes. Int J Pharma Sci. 3(2) 2013: 175-180
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175-180
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ABSTRACT: The Anthelmintic activities of methanolic and aqueous extracts of stems of Achyranthes aspera linn. were evaluated separately on Indian adult earthworms Pheretima posthuma (Annelida). For this work the stems were extracted separately with methanol and distilled water by following maceration method. Various concentrations (2.5, 5, 10, 20 mg/ml) of each extract were tested for anthelmintic activity which involved the determination of time of paralysis and time of death of the worms. The phytochemical screening of the crude extract showed the presence of saponins A and B, terpenoids, volatile oils. All extracts were able to show anthelmintic activity at all concentrations. The activities are well comparable with the standard drug, Albendazole as positive control. All the methanolic extracts showed better anthelmintic activity than the standard drug as well as the aqueous extract. Tween 20 (1%) with saline was used as negative control, did not showed any anthelmintic activity.
KeyWords: Achyranthes aspera linn., Pheretima posthuma, Anthelmintic activity, Methanolic extract,
Aqueous extract.
How to cite: Naga Bharathi M et al. In-vitro Anthelmintic Activity of Methanolic and Aqueous Extracts of Achyranthes aspera linn. (Amaranthaceae) Stems. Int J Pharma Sci. 3(2) 2013: 181-184
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ABSTRACT: Since many years, plants were known to possess anticancer activities against different cancer cell lines. In this paper, we report a study based on anticancer properties of three plants Rubia cordifolia, Plumbago zeylanica, Calophyllum inophyllum respectively. The leaves of plant material were collected, shade dried and extracted with methanol using soxhlet extraction procedure. Anticancer activities are assayed with standard MTT colorimetric procedure against MCF-7 and HT-29 cell lines. From the analysis it was found that Rubia cordifolia and Plumbago zeylanica showed nearly 50 % MCF-7 cell line inhibition at 200µg/ml tested dose, where as three plant species did not display much anticancer activities against HT-29 colon cancer cell line. Further work is in progress to evaluate the chemical constituents present in these plants.
KeyWords: Rubia cordifolia, Plumbago zeylanica, Calophyllum inophyllum, MTT assay, MCF-7, HT-29.
How to cite: Sankara Aditya J et al. In vitro anti-cancer activities of few plant extracts against MCF-7 and HT-29 cell lines. Int J Pharma Sci. 3(2) 2013: 185-188
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185-188
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ABSTRACT: A simple reverse phase HPLC method was developed for the determination of Repaglinide present in pharmaceutical dosage forms. A Discovery HSF5 C18, 4.6mm x 250 mm, 5 µm column from Supelco (India), with mobile phase methanol: ammonium acetate buffer (pH-4) (80:20) was used. The flow rate was 1.0 ml/min and effluent was monitored at 240 nm. Ritonavir is used as internal standard. Calibration standards were prepared by spiking working standard solutions into methanol containing 5 mL volumetric flasks to yield concentrations of 0.5, 1, 2, 5, 7.5, 10, 15, 20, 50, 100, 150, and 200 μg /mL. The retention times were 6.2 min and 5.2 min for REP and RIT respectively. The linearity range was found to be 0.5-200 g/ml for REP. The proposed method was also validated.
KeyWords: Repaglinide , Ritonavir, Retention time.
How to cite: Pritosh Pattanaik et al. New RP-HPLC Method for the Estimation of Repaglinide in Bulk and in Pharmaceutical Dosage Forms. Int J Pharma Sci. 3(2) 2013: 189-193
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189-193
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ABSTRACT: A simple, rapid reverse-phase high performance liquid chromatographic method has been developed and validated for the drug Sildosin in pure and in capsule dosage form. The estimation was carried out on a Phenomenax Luna C18 (150mm × 4.6 mm i.d., particle size 5µm) column with a mixture of Phosphate buffer and Acetonitrile with a pH 3.0 adjusted with ortho phosphoric acid in the ratio of 40:60%v/v. UV detection was performed at 219nm. The method was validated for linearity, accuracy, precision, specificity and sensitivity as per ICH norms. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form. The retention time was 2.32 min. and the flow rate was 0.8 ml min -1. The calibration curve was linear over the concentration range of 50-90 µ g mL-1. The LOD and LOQ values were found to be 2.93 and 9.91. The high percentage of recovery and low percentage coefficient of variance confirm the suitability of the method for estimation of Sildosin in pharmaceutical dosage form.
KeyWords: Sildosin, RP-HPLC, Phosphate buffer, Acetonitrile.
How to cite: Mohan Goud V et al. Method Development and Validation of RP-HPLC Method for assay of Sildosin in Pharmaceutical Dosage Form. Int J Pharma Sci. 3(2) 2013: 194-196
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194-196
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ABSTRACT: A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for ZOLMITRIPTAN. Chromatographic analysis was performed on Column Symmetry C18 (4.6 x 150mm, 5 m, Make: Thermosil) mobile phase employed was a mixture of buffer and organic solvent at a specific flow rate and identified by UV detector. The method was validated for accuracy, precision, specificity, linearity, and robustness. The retention time of Zolmitriptan was found to be 2.460±0.137 respectively. Linearity was observed in concentration ranges of 30–70 µg/ml. The limit of detection and the quantification limit were found to be within limits. The accuracy of the proposed method was determined by recovery studies and found to be 99.4%.
KeyWords: Zolmitriptan, Triptans, RP-HPLC, Linearity, LOD & LOQ.
How to cite: Pragati Ranjan Satpathy et al. Development and Validation of RP-HPLC method for the assay of Zolmitriptan. Int J Pharma Sci. 3(2) 2013: 197-200
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197-200
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ABSTRACT: Inverse gas chromatography (IGC) represents a unique refinement of a classic chromatographic method has the potential to unlock some of the more difficult to measure physicochemical properties of pharmaceutical materials such as powder surface energies, acid/base/polar functionality of surfaces, diffusion kinetics, solubility parameters, surface heterogeneity and phase transition temperatures/humidities. In IGC the roles of the stationary (solid) and mobile (gas or vapor) phases are inverted from traditional analytical gas chromatography (GC). In IGC, a single gas or vapor (probe molecule) is injected into a column packed with the solid sample under investigation. Instead of an analytical technique, IGC is considered a materials characterization technique.
KeyWords: Inverse gas chromatography, gas chromatography.
How to cite: M. Shyamala et al. Pharmaceutical Applications of Inverse Gas Chromatography. Int J Pharma Sci. 3(2) 2013: 201-204
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201-204
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ABSTRACT: A simple fast, accurate, precise and cost effective isocratic RP-UPLC method is developed for simultaneous determination of Esomeprazole and Naproxen in tablet formulation. The retention times of Esomeprazole and Naproxen were found to be 0.7 and 1.2 minutes respectively. The method was linear over the range of 5 to 25 ppm with correlation of 0.999 for Esomeprazole and 125 to 625 ppm with correlation of 0.998 for Naproxen. Mean recovery for Esomeprazole and Naproxen were 99.82% and 99.74% respectively. The method found simple, accurate, precise, linear over the given range, rugged and robust.
KeyWords: Esomeprazole and Naproxen, RP-UPLC, PDA detector.
How to cite: T. Manikya Rao et al. Stability indicating assay of Esomeprazole and Naproxen in Tablets by RP-UPLC PDA-Method. Int J Pharma Sci. 3(2) 2013: 205-210
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205-210
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