Current Issue
International Journal of Pharma Sciences is an open access bi-monthly journal covering all aspects of latest research in pharmacy and pharmaceutical sciences.
2017
Volume 7, Issue-5 (September-October 2017)
....
...........in progress
ABSTRACT: Pluripotent medicinal plants and their derivatives were famous spices and flavoring ingredients widely used in cooking and can be incorporated into different varieties of lifestyle as antioxidants and antimicrobial agents. The purpose of this project was to investigate the inhibitory properties of oak, cinnamon, oregano and thyme extracts on multidrug resistant and biofilm producing Klebsiella pneumoniae strains isolated from clinical mastitis cases in Baghdad. The statistical and scientific designing of this project depends on detection of ESBL producing K. pneumoniae strains from mastitis cases in regions of Abu-Ghraib, Al-Fudhaliyah and Al-Sadrya; via culturing and biochemical series confirmation tests. Extract disc sensitivity test, Kirby-Bauer technique using Carbapenems (Imipenem and Meropenem) with Oxoid Cefpodoxime Combination double diffusion inhibition kit using Muller-Hinton agar and McFarland turbidity tubes for checking antibiotic susceptibility pattern of isolates depending on instructions of USFDA Clinical Laboratory Standards Institute (CLSI). Preparation of crude concentrated and diluted, double distillated or purified extracts of locally collected and imported oak, cinnamon, oregano and thyme barks, leaves and nuts from different regions in Baghdad as watery and oily or alcoholic extracts according to recommendations of Codex international standards of Association of Official Agricultural Chemists (AOAC). Totally collected milk samples from mastitic Cows were thirty: ten samples from each region from January to April (2017), in which they processed according to modified food microbiological methods. Serum bactericidal power, minimum inhibitory concentration and minimum bactericidal concentration (MIC & MBC), Haemolysis pattern and Siderophore activity were checked using modified procedures. Chi-square analysis via SPSS software was applied for checking significant differences. The results revived detection of five (16.66%) isolates of K. pneumoniae out of thirty samples: three (10%) from Abu-Ghraib region and one (3.33%) from each Al-Fudhaliyah and Al-Sadrya regions, in which ESBL producers were only detected from Abu-Ghraib cases as totally three (10%) isolates themselves. Pluripotent Oak (Baloot), Cinnamon, Oregano and Thyme Extracts exhibit inhibitory effects on isolated strains of K. pneumoniae compared to selected antibiotics. These selective toxicity properties reflex their active ingredients that interacts directly and indirectly in different mechanisms with electromagnetic cloud (biofilm complex layers) of these multidrug resistant clinical isolates.
KeyWords: Oak, Cinnamon, Oregano, Thyme, Klebsiella pneumoniae, Biofilm, ESBL, Mastitis.
How to cite: Ali Hassan Ahmed Al-Shammary et al. The Effect of Oak, Cinnamon, Oregano and Thyme Extracts on Biofilm producing ESBL Klebsiella pneumoniae. Int J Pharma Sci. 7(5) 2017: 1839-1847
ABSTRACT: Myocardial infarction (MI) is characterized by an inequity of coronary blood supply and demand, which results in myocardial ischemic injury and damages the cardiomyocytes. Isoproterenol (ISO) is a synthetic catecholamine found to cause toxicity leading to severe stress in the myocardium of experimental animals. The aim of the present article is to investigate the cardioprotective activity of cranberry extract against ISO-induced cardiotoxicity in adult rats. Oral administration of cranberry extract at a concentration of 75 and 150 mg/kg b.wt. daily for 28 days showed a significant protection against-induced alteration in plasma total cholesterol (TC), triacylglycerols (TG), high density lipoprotein (HDL-C), low density lipoprotein (LDL-C), tumor necroses factor-alpha (TNF-α) and nitric oxide (NO) as well as cardiac superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), catalase (CAT) levels. In addition, cranberry extract reduced plasma Creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) as well as cardiac thiobarbituric acid reactive substances (TBARS) levels as compared to control group. In conclusion, cranberry extract renders resiliency against isoproterenol cardiotoxicity due to its antioxidant and free radical scavenging activity.
KeyWords: Cranberry extract, isoproterenol, myocardial infarction, antioxidant and free radical scavenging activity.
How to cite: Abdel-Maksoud A. Hussien et al. Cardioprotective effect of Cranberry extract on isoproterenol-induced myocardial infarction in rats. Int J Pharma Sci. 7(5) 2017: 1848-1854
