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International Journal of Pharma Sciences is an open access bi-monthly journal covering all aspects of latest research in pharmacy and pharmaceutical sciences.
2019
Volume 9, Issue-2 (March-April 2019)
ABSTRACT: The present study was conducted to evaluate the protective effect of Spirulina on mycotoxins induced liver damage and oxidative stress in rats. Thirty male albino rats were classified randomly into three equal groups. Group1(normal control): Rats fed with ordinary diet. Group2 (Mycotoxin): Rats fed with ordinary ration only for first 7 days and from day 8 rats fed mixed ration contain 50 gram of grinded plant (total aflatoxins 4.2 ppb) + 50 gram of grinded fish (total aflatoxins 42.14 ppb) + 50 gram of ordinary diet (free of aflatoxins) for 4 weeks. Group3 (Mycotoxin+ Spirulina): Spirulina platensis (500 mg/kg b.wt/day) was administered orally all over the experimental period (5 weeks). Also, rats were fed on ordinary diet only for first 7 days and from day 8 rats fed mixed ration as that mentioned above in group 2. At the end of the experiment liver tissue specimens were isolated and analyzed for the determination of reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), L-malondialdehyde (L-MDA) in addition to intrerleukin-1(IL-1) gene expression and DNA damage. The obtained results showed a significant up-regulation of IL-1 gene expression level and marked increase in SOD, CAT, L-MDA and DNA damage that was indicated by an increase in tail length and tail DNA % in liver tissue of mycotoxin rats group. However, liver GSH concentration was markedly decreased as compared to control group. Spirulina protected mycotoxins induced liver damage in rats caused a significant improvement of all previous parameters and attenuates DNA changes. Conclusively, Spirulina platensis treatment exerts a protective effect against DNA damage and oxidative stress in liver of mycotoxin intoxicated rats through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidants defense system mechanisms.
KeyWords: Mycotoxins, liver damage, Spirulina platensis, oxidative stress.
How to cite: Samy A. Hussein et al. Attenuating Effect of Spirulina platensis against Mycotoxin Induced Oxidative Stress and Liver Damage in Male Albino Rats. Int J Pharma Sci. 9(2) 2019: 2039-2044
ABSTRACT: Moringa oleifera leaves have therapeutic functions such as antioxidant, anti-bacterial, anti-viral, anti-cancer, anti-inflammatory, anti-allergic and anti-diabetic. The purpose of this study was to evaluate the protective effect of Moringa oleifera on obesity induced oxidative stress and biochemical abnormalities in male rats. Forty-eight male albino rats were divided into three equal groups. Group I (control normal): rats fed normal diet. Group II (Obesity): rats received high fat diet (HFD).Group III (Obesity+ Moringa oleifera): rats received Moringa oleifera (600 mg/kg.b.wt.) orally for two months after induction of obesity. The obtained results showed significant increase in levels of serum insulin, lipids profile (total cholesterol and triacylglycerols), liver L-MDA in addition to up regulation of Leptin and Nuclear factor kappa B (NF-κB) gene expression level in obese rats. However, liver catalase activity and GSH concentration were markedly decreased. Moringa oleifera treatment to high fat diet-induced obesity in rats caused significant improvement of all previous parameters towards its normal ranges. These results suggested that, Moringa oleifera treatment exerts a protective effect on obesity by reduction of oxidative stress markers, inflammation and hyperlipidemia in rats through free radical scavenging and anti-inflammatory activities as well as regenerating endogenous antioxidant defense system mechanisms.
KeyWords: Obesity, Moringa oleifera, oxidative stress, leptin, NF-κB.
How to cite: Samy A. Hussein et al. Moringa oleifera ameliorates lipid metabolic disorders, oxidative stress and the inflammatory status in high fat diet-induced obesity in rats. Int J Pharma Sci. 9(2) 2019: 2045-2052
