Current Issue
International Journal of Pharma Sciences is an open access bi-monthly journal covering all aspects of latest research in pharmacy and pharmaceutical sciences.
2017
Volume 7, Issue-3 (May-June 2017)
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...........in progress
ABSTRACT: In severe illness of cancer cause, depression of the thyroid hormone system may occur in otherwise euthyroid patients, Sixty five specimens of blood were collected from 65 patients with thyroid cancer and 50 healthy between 30 and 48 years old attending the thyroid centre in Al yarmook teaching hospital from the period November 2014 to June 2015. All samples were inoculated to study different characteristics of hormones. The blood was collected for hormonal assay the results revealed a gradual decrease in serum TT3 levels with a concomitant increase in Serum Thyroid stimulating hormone TSH level when compared with control healthy and also a reduction in TT4 in thyroid cancer patients compared with control healthy.
KeyWords: Thyroid cancer; hormonal identification.
How to cite: Madlen Qassm abbas. Evaluation of Various Thyroid Hormones on The patients with Thyroid cancer. Int J Pharma Sci. 7(3) 2017: 1760-1764
ABSTRACT: The present study aimed to evaluate the antioxidant and hepatoprotective effects of Honey and Propolis against liver injury induced by Ethanol. Female Sprague dawley rats were used, rats were injected orally With Ethanol (10%), daily for 6 weeks and treated orally with Honey(150mg/kg) & Propolis (200mg/kg) daily for 6 weeks and compared with a group of rats , as a control group. Liver functions, Glutathione activity (Gpx), blood reduced glutathione (GSH) and malondialdehyde (MDA) in red blood cells were determined. Moreover TNF-α and IL-2 Cytokines in serum were also assayed in addition to liver histopathology. Liver enzymes, total billirubin, MDA and cytokines were increased after injection with ethanol, otherwise serum albumin, total protein, as well as blood glutathione level were reduced. Treatment with honey & propolis reduce the level of MDA and activity of liver enzymes and increase the activity of the antioxidant parameters. Simultaneously, the histological deteriorative effects of ethanol intoxication were also improved after treatments . In conclusion, Honey and propolis enhanced innate antioxidant activity and ameliorate the ethanol-induced liver injury and therefore can be used as a hepatoprotective agent in the future.
KeyWords: Honey; Propolis; Ethanol; Fibrosis; Liver.
How to cite: Kadry El-bakry et al. The effects of bee products on liver and kidney fibrosis of rats. Int J Pharma Sci. 7(3) 2017: 1765-1771
ABSTRACT: It was debatable whether cadaveric dissection is the more indicated method for the learning of Medical Sciences, while there has been a vast increase in claims associated with the lack of medical knowledge. Problem-based learning (PBL) helps students develop their knowledge and understanding skills. This study aims to compare the effect of PBL and lecture-based learning (LBL) on examination results of undergraduate medical students of Medical Laboratory program, Faculty of Applied Medical Sciences, October 6 University, Egypt. This study was conducted in Clinical chemistry, immunology, molecular biology, epidemiology and nutrition courses at department of medical laboratories, Faculty of Applied medical Sciences, October 6 University, Egypt, at the first semester of 2016-2017 academic year. A total of 127 students were selected and they were divided into two groups as the LBL group (n= 109) and the PBL group (n=19). The pass, good, very good and excellence rates and examination scores of courses examination were collected. Methodological quality was evaluated based on the percentage ratio. The present study included 127 students and 5 courses (Clinical chemistry, immunology, molecular biology, epidemiology and Nutrition). A very significant improvement was observed among the students after the PBL sessions and they were also motivated by these sessions. The PBL model yielded higher course examination pass rates [23.5%], very good rates [41.1%], excellence rates [35.5%] and examination scores [94.4%] for clinical chemistry course. Also, the scores of students exposed to immunology are pass rates [12.5%], good rates [18.7%], very good rates [36.5%], excellence rates [31.3%] and examination scores [88.8%]. However, The PBL model yielded higher course examination pass rates [22%], good rates [16.6%], very good rates [27.7%], excellence rates [33.3%] and examination scores [100%] for Molecular biology course. In addition, the scores of students which exposed to epidemiology are pass rates [21.4%], good rates [14.3%], very good rates [35.7%], excellence rates [28.5%] and examination scores [77.7%]. Finally, The PBL model yielded higher course examination pass rates [0%], good rates [6.3%], very good rates [68.7%], excellence rates [25%] and examination scores [88.8%] for molecular biology course. PBL teaching model application is effective in the medical curriculum for a better understanding of Clinical chemistry, Immunology, Molecular biology, Epidemiology and Nutrition courses among the students in Faculty of Applied medical sciences, October 6 University.
KeyWords: Problem-based learning, clinical biochemistry, Applied medical sciences, October 6 University.
How to cite: Ismail H and Hussein MA. Problem Based Learning: A Modern teaching and learning of Clinical Medical Sciences in Health Professions’ undergraduate curriculum. Int J Pharma Sci. 7(3) 2017: 1772-1779
ABSTRACT: The ameliorative effect and antitumor activity of basic curcumin nanoparticles modified with basic nano black seeds (Nigella sativa) and calcium ascorbate were evaluated. Eighty female mice were divided into 4 equal groups of 20 mice each . Group 1 : non – tumor bearing mice (NTBM) . Group 2 : tumor bearing mice(TBM) . Group 3 : NTBM + nanocomposite (4.750gm / kg. b.wt /day ) , orally for 6 weeks . Group 4 : TBM + nanocomposite ( 4.750gm / kg. b.wt/day ), orally for 6 weeks . Blood samples for serum seperation and liver tissues specimens were collected from all animal groups three times after 2, 4 and 6 weeks from the onset of treatment. All serum were directly used for determination of ALT and AST activities and urea and creatinine concentrations. Moreever, antioxidants enzymes (catalase , GPX , SOD ), GSH and MDA were detected in liver tissue . The obtained results showed significant decrease in catalase, GPX and SOD activities and GSH concentration in liver tissue of tumor bearing mice. Meanwhile, liver MDA, serum transferases(ALT and AST) activities, urea and creatinine concentrations were significantly increased in tumor bearing mice . TBM treated with nanocomposite improve the adverse biochemical changes induced by tumor. So, this nanocomposite has potential benefits in cancer treatment.
KeyWords: nanocomposite, curcumin nanoparticles, black seed nanoparticles, antioxidants enzymes, hepatorenal functions, oxidative stress.
How to cite: Omayma.A.Ragab Abou Zaid et al. Ameliorative effect of novel nanocomposite, modified with basic nano black seeds (Nigella sativa) and calcium ascorbate on hepatic oxidative stress and antioxidant status in tumor of female mice. Int J Pharma Sci. 7(3) 2017: 1780-1786
ABSTRACT: The proposed study, a new stability- indicating RP-HPLC method has been developed for estimation of Sacubitril and Valsartan in bulk and tablet dosage form. The present method was a sensitive, precise, and accurate RP-HPLC method for the analysis of Sacubitril and Valsartan. To optimize the mobile phase, various combinations of buffer and organic solvents were used on Kromasil-(250x4.6mm, 5µ) column. Then the mobile phase containing a mixture of Perchloric acid: Acetonitrile in the ratio of 50:50% v/v was selected at a flow rate of 1.0 ml/min for developing the method and the peaks with good shape and resolution were found resulting in short retention time, baseline stability and minimum noise. The retention times of Sacubitril and Valsartan were found to be 2.438min and 3.709min respectively. Quantitative linearity was obeyed in the concentration range of 24.25-145.25 and 25.8-154.8 µg/mL of Sacubitril and Valsartan respectively. The limit of detection and limit of quantification were found to be 0.090 µg/mL and 0.274 µg/mL (Sacubitril); 0.06 µg/ mL and 0.19 µg/mL (Valsartan) respectively, which indicates the sensitivity of the method. The high percentage recovery indicates that the proposed method is highly accurate. No interfering peaks were found in the chromatogram indicating that excipients used in injection formulations didn’t interfere with the estimation of the drugs by the proposed HPLC method.
KeyWords: RP-HPLC, Sacubitril, Valsartan, Validation.
How to cite: B. Madhu Harika et al. Development and Validation of Stability Indicating RP-HPLC method for simultaneous estimation of Sacubitril and Valsartan in bulk and tablet dosage form. Int J Pharma Sci. 7(3) 2017: 1787-1793
ABSTRACT: The main objective of this study to investigate the alterations of chitosan on Hepatic function tests and Immunoglobulin in Experimental induced Nonalcoholic fatty liver disease [NAFLD] in rats. Thirty male albino rats were divided into three groups [10 rats each]. The first group fed a normal diet and represents the control group. The second group [NAFLD] fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive control [+ve control]. The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and chitosan to the basal diet at a dose 5%. Samples collected after 2,4and6 weeks after induction from treatment .serum was used for estimation of Liver Function Tests as Serum Aspartate aminotransferase [AST], Alanine aminotransferase [ALT], Gammaglutamyltransferase [GGT] and Immunoglobulins as Serum ImmunoglobulinA [IgA], Immunoglobulin G [IgG]. Our Results revealed a significantly increase in serum AST, ALT, GGT, Serum IgA, IgG were significantly increase was observed. The behavioral biochemical results indicated treatment with chitosan showed significant changes and improves these parameters.
KeyWords: NAFLD, Chitosan, Liver Function Tests, Immunoglobulins.
How to cite: Omayma.A.Ragab Abou Zaid et al. Biochemical alterations of Chitosan on Liver function tests and Immunoglobulin in Experimental induced Non Alcoholic Fatty Liver Disease in Rats. Int J Pharma Sci. 7(3) 2017: 1794-1798
ABSTRACT: The main objective of this study to investigate the protective effect of probiotics on Immunoglobulin and liver function tests In Experimental Induced Nonalcoholic fatty liver disease [NAFLD] in rats. Thirty male albino rats were divided into three groups [10 rats each]. The first group fed a normal diet and represents the control group. The second group [NAFLD] fed normal diet enriched with1% cholesterol and 2% coconut oil and act as positive group [+ve control]. The third group fed on normal diet enriched with1% cholesterol and 2% coconut oil and probiotics [BIO-BC™] at a dose of 1-2g/liter at a rat dose [0.5-1ml/kg. body. Weight] orally. Samples collected after 2,4and6 weeks after induction from treatment. serum was collected for estimation of Immunoglobulins as Serum ImmunoglobulinA [IgA] Immunoglobulin G [IgG] and Liver Function Tests as Serum Aspartate aminotransferase [AST], Alanine aminotransferase [ ALT], Gamma glutamyltransferase [GGT]. Our Results revealed a serum AST, ALT, GGT were significantly increase concentration In addition to Serum IgA, IgG were significantly increase was observed. The behavioral biochemical results indicated treatment with probiotics showed significant changes and improved these parameters.
KeyWords: NAFLD, probiotics, Immunoglobulin, Liver Function Tests.
How to cite: Omayma.A.Ragab Abou Zaid et al. Protective effect of probiotics on Immunoglobulin and Liver function tests in Experimental induced Non Alcoholic Fatty Liver Disease in Rats. Int J Pharma Sci. 7(3) 2017: 1799-1802
